Everything about the lab industry

As a trust-worthy chemical supplier, Alfa Chemistry has been producing THEIC for over 25 years and has hence accumulated a wealth of experience in this field. With its advanced production line, state-of-the-art production technologies and efficient internal management, the company announces to be capable of producing 20,000 tons of THEIC annually. THEIC (CAS No. 839-90-7), with the chemical name of 1,3,5-Tris(2-hydroxyethyl)cyanuric acid, is mainly used as an important raw material for the production of wire enamels. Owing to its excellent performance in heat resistance, flexibility, adhesiveness, hardness, and glossiness, it is also used as PVC heat stabilizer. Besides, THEIC can also be used in the production of various polyurethane plastics, water-based baking lacquer, unsaturated polyester resins, pesticides, water-soluble paints for electrodeposition coating, general oil-based paints, pharmaceutical intermediates as well as other aspects. Three major applications of THEIC: Cross-linking agent for insulating varnish for wire enamels Stabilizer for PVC Painting and coating Statistics show that electrical insulation made with THEIC polyesters exhibit good dielectric properties in regard to adhesion, flexibility, heat shock, and heat aging. The THEIC–MR-phosphate improved the heat resistance of PVC materials. Paintings and coatings made with THEIC also exhibit good mechanical properties such as improved heat resistance and weather resistance. "Our THEIC is sold well in more than 20 countries and regions worldwide, including Europe, America, China, Japan, Korea, and Southeast countries etc. There are different packages for choice, for example, 25kg two-ply plastic bags or 500kg/800kg anti-static bags with inner plastic bags. THEIC should be stored in dry and sealed condition, and be protected from moisture," says a spokesperson from Alfa Chemistry. All chemicals provided by Alfa Chemistry are strictly QC assessed so that high quality is guaranteed. Technique documents such as COA are also provided. For more information about THEIC or any other chemicals, please visit the website or email us to discuss with our experts. About Alfa Chemistry Alfa Chemistry is a reliable supplier of various chemicals widely recognized by pharmaceutical companies, universities and research institutions. To meet the most recent market demand, the company is now expanding and optimizing its product lines. Some newly emerging or rare chemicals that can be hardly found elsewhere are now available at Alfa Chemistry as well.

FMT is a hot research field in biological therapy in recent years and is used in the treatment of gastrointestinal diseases such as inflammatory bowel disease, autoimmune bowel disease, irritable bowel syndrome, and clostridium difficile enteritis. And it is also a new therapeutic research direction for diseases such as Parkinson's disease, chronic fatigue syndrome, multiple sclerosis, obesity, metabolic syndrome, and major depression. Creative BioMart Microbe has isolators and an experimental system that can be used for sterile mouse research, and has the ability to provide customers with suitable animal models. In addition, there are many types of microorganisms in the feces, and the analysis of the microbiota will be involved in the study. Creative BioMart Microbe provides a number of technical services for FMT researchers to help customers greatly reduce the research cycle. Services for Fecal Microbiota Transplantation at Creative BioMart Microbe FMT Technical Outsourcing Service HFA Mice Model Construction Sterile Mice Customized Service Fecal Microbiota Test Service Advantages * Complete microbial analysis technology platform, including microbial metabolomics analysis platform, metagenomics sequencing and metatranscriptomics sequencing technology platform, etc. * Excellent technical team and rich experience in FMT * Equipped with a laboratory that meets the conditions for feeding sterile mice and a standardized test system for sterile mice * More competitive prices in the same industry “We have an experienced team of experts and have established a technology platform to provide one-stop microbiological services to help customers solve the problems they encountered in their research.” commented Linna, the chief marketing staff at Creative BioMart. Creative BioMart Microbe, a sub-brand of Creative BioMart that provides microbiological services and products to multi-industry customers, now has been focusing on the development of microbiotherapy, especially researches related to fecal microbiota transplantation, trying to help customers engaged in FMT researches to solve difficulties.

When cultivating microorganisms in a laboratory, a growth environment called a culture medium is used. The culture medium may be pure chemical substances (medium with a determined chemical composition), or it may contain organic substances, or it may be composed of living organisms such as fertilized eggs. Microorganisms that grow in or on such media form cultures. If there is only one type of organism, the culture is regarded as a pure culture, and if there are populations of different organisms, it is regarded as a mixed culture. When used for the first time, the culture medium should be sterile, which means that there are no life forms before the inoculation of the microorganisms. General microbial culture medium For bacterial culture, the commonly used medium is nutrient broth, which is a liquid containing protein, salt and growth promoters, which can support many bacteria. In order to solidify the medium, add agar and other reagents. Agar is a polysaccharide, it does not add nutrients to the medium, but only solidifies it. The resulting medium is nutrient agar. Many media for microorganisms are complex and reflect the growth requirements of microorganisms. For example, most fungi require additional carbohydrates and an acidic environment to achieve optimal growth. The medium used for these organisms is potato dextrose agar, also known as Sabouraud dextrose agar. For protozoa, liquid culture medium is generally required. For rickettsiae and virus, living tissue cells must be provided for optimal culture. For anaerobic microorganisms, the atmosphere must be anaerobic. To eliminate oxygen, the culture medium can be placed in a container that produces carbon dioxide and hydrogen and removes oxygen from the atmosphere. Commercial products meet these conditions. Anaerobic chambers can also be used in closed compartments where technicians can manipulate the culture medium. In order to promote the formation of carbon dioxide, candles can be burned to consume oxygen and replaced with carbon dioxide. Special microbial culture medium Certain microorganisms are cultivated in selective media. These media prevent the growth of unwanted organisms while promoting the growth of desired organisms. For example, mannitol salt agar is selective for staphylococci because most other bacteria cannot grow in its high salt environment. Another selective medium is Bright Green Agar, which inhibits Gram-positive bacteria while allowing the growth of Gram-negative bacteria such as Salmonella. There are other cultural media that are different media. These media provide an environment in which different bacteria can be distinguished. For example, Fuchsia Bile Agar is used to distinguish coliforms, such as Escherichia coli and non-coliforms. The coliforms are bright pink colonies in this medium, while the non-coliforms are pale pink or transparent. Certain media are both selective and differentiated. For example, MacConkey Agar distinguishes lactose-fermenting bacteria from non-lactose-fermenting bacteria, while inhibiting the growth of gram-positive bacteria. Since lactose-fermenting bacteria are often associated with water pollution, they can be distinguished by adding a water sample to MacConkey agar and waiting for growth to appear. In some cases, it is necessary to prepare enriched media. This medium provides specific nutrients and can promote the reproduction of specific types of microorganisms in mixed samples. For example, when trying to isolate Salmonella from a stool sample, it is helpful to place the material sample in an enriched medium to promote the propagation of Salmonella before the isolation technique begins. In order to use microorganisms in the laboratory, they need to be obtained in pure culture. A pure culture of bacteria can be obtained by spreading the bacteria and allowing individual cells to form growing clumps called colonies. You can then pick a sample from the colony and make sure it contains only one type of bacteria. Culturing these bacteria on a separate medium will produce a pure culture. In order to preserve microbial cultures, they can be placed in the refrigerator to slow down the rate of metabolism. The other two methods are deep freezing and freeze drying. For deep freezing, microorganisms are placed in a liquid and quickly frozen at a temperature below –50°C. Freeze-drying (lyophilization) is carried out in equipment that uses vacuum pumping after freezing the microbial suspension. The culture is similar to a powder, and the microorganisms can be stored for a long time under this condition.

Successful recombinant protein expression and purification are often essential for basic research as well as biotechnology and commercial applications. High-throughput protein expression and purification in Escherichia coli has begun to revolutionize the research methods in various research fields. Experiments that are usually performed manually are processing one protein at a time in a few weeks, and now hundreds of proteins can be performed in just one week. However, limitations still exist, and further improvements are possible. The ease of genetic manipulation, low cost, rapid growth and the number of previous studies have made E. coli one of the most widely used microbial species for the production of recombinant proteins. In this post-genomic era, rapid expression and purification of large numbers of proteins for academic and commercial purposes in a high-throughput manner is still facing challenges. At present, several state-of-the-art methods are suitable for the cloning, expression and purification of a large number of molecules, and the latest developments related to soluble protein expression, mRNA folding, fusion tags, post-translational modifications and membrane protein production are discussed. HTP High-throughput research can be defined as research that allows the measurement results of thousands of biomolecules to be obtained at the same time, thus making large-scale repetitions possible. In the post-genomic era, the use of high-throughput technologies for measuring DNA, RNA, proteins, lipids, and metabolites has increased dramatically. These technologies have been successfully applied to answer various biological questions related to cancer biology. Protein expression and purification play a central role in biochemistry. Recombinant proteins can be expressed using prokaryotic systems (E. coli and Bacillus subtilis), eukaryotic systems (yeast, insect cells and mammalian cells) or in vitro systems. The E. coli system is the host of choice for preliminary screening of recombinant protein expression, because these cells can be easily manipulated, cultivated inexpensively, and grow rapidly. In recent years, many new strains, vectors, and tags have been developed to overcome the limitations of the system, including codon bias, inclusion body formation, toxicity, protein inactivation, mRNA instability, and lack of post-translational modifications. It has been extensively studied in the E. coli expression system, but it is labor-intensive and time-consuming to use this system for protein expression and purification. Therefore, parallel, high-throughput methods must be used for protein expression and purification, which has always been the bottleneck of protein function, structure and application research in the post-genomic era. High-throughput protein production methods are widely used, and recombinant proteins in the form of inclusion bodies are even expressed and purified in a parallel manner. Effective promoter An effective promoter for expressing heterologous proteins in E. coli has four key features: First, the promoter is strong enough to allow the accumulation of recombinant protein to be greater than or equal to 10-30% of the total cell protein; second, it shows the minimum basic transcriptional activity, so unnecessary transcription is avoided before induction; third, the promoter can be induced simply and cheaply; fourth, the promoter activity can be precisely adjusted. The selection of strains for expressing recombinant proteins also plays an important role in protein expression, solubility and yield. A few E. coli strains such as BL21 and its derivatives are widely used. Different E. coli strains promote the expression of proteins containing disulfide bonds or proteins encoded by genes that contain rare codons and proteins that are toxic to E. coli. In addition, co-expression with some genes increased the expression of post-translationally modified proteins. So far, several E. coli strains that can significantly increase membrane protein production have been designed