Enzyme is a large category of bio-molecules that catalyze various biological processes including metabolic processes, cellular signaling and regulation, cell division and apoptosis. Enzymatic reactions convert substrate molecules into chemically modified molecules products with high specificity and accuracy. These modifications include proteolytic digestion, glycolytic degradation, phosphorylation, acylation, glycosylation or oxidative and reductive processes.
As play important roles in numerous physiological activities, many of them become critical targets for diseases treatments. For example, matrix metalloproteinase (MMP) plays critical role in anti-tumor metastasis. Protein kinase B (PKB) and protein kinase C (PKC) are major drug targets for clinical treatment of diabetes. HIC and HCV viral proteinase can serve as therapeutic target for antiviral therapy. Cytochrome P450 is a vital enzyme during drug metabolism and has great influence on thermoregulation. Based on the above, enzyme activity assay can bring great significance in novel drug development and clinical therapy.
