Best Practices for Fluorescence Imaging I Evident I Analis I - Labinsights

Best Practices for Fluorescence Imaging I Evident I Analis I

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11 June 2025
Best Practices for Fluorescence Imaging
Best Practices for Fluorescence Imaging | Photo: Analis sa/nv
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Neurofilament-heavy chain (NFH) in green, myelin basic protein (MBP) in red, glutathione S-transferase pi 1 (GSTpi) in blue. Mouse cerebellum captured with a UPLXAPO40X objective. | Photo: Analis sa/nv
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FLUOVIEW FV4000 Confocal Laser Scanning Microscope SKU: fv4000 | Photo: Analis sa/nv
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Evident-fv4000-truspectral-drosophila-leg-imaging | Photo: Analis sa/nv
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Evident-Fluoview-FV4000-SilVIR-Detector-Components-and-Sensitivity | Photo: Analis sa/nv
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fv4000-truspectral-multiplex-imaging-6-channel | Photo: Analis sa/nv
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Cytoskeleton sample: HeLa cells stained with DAPI (blue), Pericentrin (Centrosome, green), a-Tubulin (Microtubules, Alexa-568; red) and phalloidin (Actin, Alexa-647; magenta). | Photo: Analis sa/nv

How to enhance your imaging workflow?

  • Fluorescence imaging begins with the right equipment and the right approach.
  • Fluorescence imaging requires a delicate balancing act.

On one hand, you need to acquire a high enough signal, which you can achieve by using a longer exposure time or stronger excitation light.

On the other hand, you need to minimize phototoxicity for the sample condition by reducing the excitation time and intensity.

Explore advanced solutions like the FLUOVIEW FV4000 confocal laser scanning microscope:

With the FV4000 confocal microscope, researchers can acquire higher-quality, quantitative image data in less time and with less effort.

It’s an advanced imaging technology that enables higher precision images to empower researchers with more reliable data from their samples. With our breakthrough SilVIR detector at the core of the system, achieve much lower noise, higher sensitivity, and improved photon resolving capabilities. With the FV4000 confocal microscope, researchers can acquire higher-quality, quantitative image data in less time and with less effort.

Contact our expert to learn how key specifications like quantum efficiency, sensor type, and cooling strategies influence your image quality.

Watch video on the Evident Scientific website.

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Analis sa/nv

Analis, distributeur & fabrikant van instrumenten en meubilair voor laboratoria. Meer dan een vertrouwenslabel, UW KWALITEITSLABEL Read more