The purification and recovery of a biological target molecule out of a tissue, cell or fermentation broth – the so called downstream processing (DSP) – usually requires a combination of separation technologies. Bioprocess chromatography is the most important technique for this purpose.
Today, biopharmaceuticals are the fastest growing product segment of the pharmaceutical industry. An effective and affordable purification of therapeutic biomolecules is key for the successful development and commercialization of new drugs, no matter whether biologic, biosimilar, or biobetter. With new biopharmaceutical formats, such as bispecific mAbs, antibody fragments and antibody-drug-conjugates (ADCs) in the pipeline, rapid development of DSP steps will become even more important.
Toyopearl®, TSKgel®, and Ca++Pure-HA® process media are renowned for their quality, reliability, and productivity, and cover all modes of bioprocess chromatography, including affinity (AFC), ion exchange (IEX), mixed-mode (MXC), hydroxyapatite, hydrophobic interac- tion (HIC), and size exclusion (SEC). They are popular in the biotech and biopharmaceutical industry and are used in R&D, method development, and production from μL to 1000-L scales.
This application notebook compiles over twenty application notes covering important aspects of biopharmaceutical purification such as capturing of antibody constructs such as Fab, scFv, or mAB, mAb aggregate removal using salt-tolerant IEC or hydroxyapatite, inter- mediate purification of bi-specific antibodies with a non-affinity platform using MXC and HIC, antibody-drug conjugates (ADC) purification on HIC resin, or separation of oligonucle- otides on highly-selective anion exchanger.
Enjoy reading and stay informed.
Read more