Redefining Precision in Molecular Biology

Redefining Precision in Molecular Biology | Photo: Creative Enzymes

Creative Enzymes unveils the SuperCut Series, expediting restriction enzyme digests, offering high precision, rapid results, and universal compatibility.

Creative Enzymes, a pioneering force in the field of biotechnology, has once again raised the bar in molecular biology with the launch of its Creative SuperCut Series. This breakthrough product line is designed to revolutionize the way scientists approach restriction enzyme digests, making it faster and more efficient than ever before.

SuperCut Series

Creative Enzymes’ Creative SuperCut Series is engineered to complete restriction digests within an impressive 5-15 minutes, a significant time-saving advantage. It is perfectly suited for applications in vector preparation, DNA fragment analysis, PCR product manipulation, and genomic DNA studies.

Reliability of enzymes

“The SuperCut Series is a testament to our commitment to innovation and excellence,” says the Creative Enzymes’ Chief Scientist. “We understand that success in molecular biology hinges on the reliability of the enzymes used. With the SuperCut Series, we are not just meeting these expectations; we are exceeding them.”

Essential features

The Creative SuperCut Series offers a range of essential features:

1. High Digestion Efficiency:
   Achieve rapid digestion, with reactions completed in just 5-15 minutes.
2. Universal Buffer:
   Utilize a universal buffer system, allowing for multiple cuts without buffer changes.
3. Compatibility:
   SuperCut enzymes work seamlessly with popular buffers such as FastDigest (Thermo Scientific), CutSmart (NEB), and QuickCut (Takara).
4. Color-Coded Buffer:
   Simplify loading and electrophoresis procedures.
5. High-Fidelity Enzymes: Ensure precise results with minimal off-target effects.

Rigorous testing

Creative SuperCut enzymes undergo rigorous testing to ensure their reliability:

1. Activity Assays:
   1 μl of SuperCut enzymes can digest 1 μg of λDNA (HindIII Digest) within 15 minutes at the optimal temperature in a 20μl system.
2. Ultra-Long Incubation Test:
   A 3-hour incubation of 1 μl SuperCut enzymes and 1 μg λDNA (HindIII digest) at the optimal reaction temperature confirms the absence of nuclease contamination or non-specific degradation, preventing star activity.
3. Digestion-Ligation-Re-Digestion Detection:
   At the optimum reaction temperature, SuperCut enzymes enable substrate digestion, followed by re-ligation with Fast T4 DNA Ligase at 22°C. Re-cutting the ligation product with the same restriction enzyme further demonstrates the enzymes’ versatility.
4. Non-Specific Endonuclease Activity Detection:
   Incubation of 1 μl of SuperCut enzyme and 1 μg of supercoiled plasmid DNA for 4 hours at the optimal temperature confirms the plasmid DNA remains in a supercoiled state.
5. Blue and White Spot Detection: SuperCut enzymes allow for efficient detection of correctly connected products through the growth of blue colonies in E. coli competent cells, while incomplete DNA end cuts result in white colonies. The proportion of white colonies in SuperCut series restriction enzymes being less than that of E. coli, with the proportion of white colonies in SuperCut series restriction enzymes being less than that of E. coli.
   1%.

Accelerated path to scientific breakthrough

Creative Enzymes extends an invitation to researchers and scientists across the globe to explore the transformative potential of the SuperCut Series. These enzymes offer new possibilities and an accelerated path to scientific breakthroughs.

About Creative Enzymes

Creative Enzymes is a remarkable supplier and manufacturer in the enzymology field. Equipped with advanced technology platforms, Creative Enzymes is able to offer high-quality and professional services to customers. Its products and services have been widely used institutionally for academia and industrially for pharmaceuticals.