Most (q)PCR optimizations are performed on DNA, primers and template variables. Yet, very little attention is paid to the effect plastics and thermocyclers have on the (q)PCR result. They are incorrectly considered to be constants in the PCR process.
In this guide we illustrate different types of thermocycler variabilities and the impact they have on the (q)PCR results. We also give practical solutions on how to eliminate or control these variabilities.
Practical
You can easily use the data from the calibration certificates in practice. We give examples on how to align and program thermocyclers to mimic each other. You also learn how to use calibration results for validation purposes.
Complimentary guide
Do you want to explore the full potential of CYCLERtest thermocycler calibration data? Click the link in the box on the right to download your complimentary copy.
